PART I NEUTROPHILS AND NEUTROPHIL DISORDERS: OVERVIEWS. -- 1 The Role of Neutrophils in the Immune System: An Overview. -- 2 Neutrophil Defects and Diagnosis Disorders of Neutrophil Function: An Overview. -- PART II NEUTROPHIL ISOLATION. -- 3 Isolation of Human Neutrophils from Venous Blood. -- 4 Isolation of Neutrophils from Nonhuman Species. -- 5 Isolation of Neutrophils from Larval Zebrafish and Their
Transplantation into Recipient Larvae for Functional Studies. -- PART III NEUTROPHIL CHEMOTAXIS, PHAGOCYTOSIS, AND
BACTERICIDAL ACTIVITY. -- 6 Analysis of Neutrophil Transmigration Through Epithelial
Cell Monolayers. -- 7 Quantification of Chemotaxis or Respiratory Burst Using Ex Vivo Culture-Derived Murine Neutrophils. -- 8 Ex Vivo Human Neutrophil Swarming Against Live Microbial Targets. -- 9 Microinjection and Micropipette-Controlled Phagocytosis
Methods for Neutrophils. -- 10 Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis. -- 11 Visualization and Quantification of Phagocytosis by Neutrophils. -- 12 Analysis of Neutrophil Bactericidal Activity. -- PART IV BIOCHEMISTRY, BIOLOGY, AND SIGNAL TRANSDUCTION OF NEUTROPHILS. -- 13 Assessment of Neutrophil Apoptosis. -- 14 Optical Methods for the Measurement and Manipulation of
Cytosolic Calcium Signals in Neutrophils. -- 15 Labeling Acidic Compartments of Neutrophils with Cresyl Violet. -- 16 Neutrophil Degranulation of Azurophil and Specific Granules. -- 17 Influence of Oxygen on Function and Cholesterol Composition
of Murine Bone Marrow-Derived Neutrophils. -- 18 In Vitro Assay for Sensitive Determination of Human Blood
PMN Responses. -- 19 Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR. -- 20 Detection of Intact Transcription Factors in Human Neutrophils. -- 21 Genome-Scale Transcript Analyses of Human Neutrophils. -- 22 Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes. -- 23 Cell-Free NADPH Oxidase Activation Assays: A Triumph
of Reductionism. -- 24 Immunofluorescent Detection of NET Components in Paraffin-Embedded Tissue. -- 25 Detection, Visualization, and Quantification of Neutrophil
Extracellular Traps (NETs) and NET Markers. -- 26 Imaging of Neutrophils and Neutrophil Extracellular Traps (NETs) with Intravital (In Vivo) Microscopy.

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